ABSTRACT
Objective: The genetic variants of the long non-coding RNA ANRIL [an antisense non-coding RNA in the INK4 locus] as well as its expression have been shown to be associated with several human diseases including cancers. The aim of this study was to examine the association of ANRIL variants with breast cancer susceptibility in Iranian patients
Materials and Methods: In this case-control study, we genotyped rs1333045, rs4977574, rs1333048 and rs10757278 single nucleotide polymorphisms [SNPs] in 122 breast cancer patients as well as in 200 normal age-matched subjects by tetra-primer amplification refractory mutation system polymerase chain reaction [T-ARMS-PCR]
Results: The TT genotype at rs1333045 was significantly over-represented among patients [P=0.038] but did not remain significant after multiple-testing correction. In addition, among all observed haplotypes [with SNP order of rs1333045, rs1333048 rs4977574 and rs10757278], four haplotypes were shown to be associated with breast cancer risk. However, after multiple testing corrections, TCGA was the only haplotype which remained significant
Conclusion: These results suggest that breast cancer risk is significantly associated with ANRIL variants. Future work analyzing the expression of different associated ANRIL haplotypes would further shed light on the role of ANRIL in this disease
Subject(s)
Humans , Female , Adult , Breast Neoplasms/genetics , Case-Control Studies , Genotype , Polymorphism, Single Nucleotide , Polymerase Chain Reaction , HaplotypesABSTRACT
Background: Ovarian cancer is the most fatal tumor of female's reproductive system, and several genetics and environmental factors are involved in its development. Various studies have already identified suitable biomarkers to facilitate the early detection, prognosis evaluation, and the assessment of treatment response. However, the aim of this review was to investigate the role of long non-coding RNAs [lncRNAs] in tumorigenesis process of ovarian cancer and their potential applications as ovarian cancer biomarkers
Methods: We performed an online literature search of the MEDLINE/PubMed databases using the key words ovarian cancer, lncRNA, and biomarker
Results: We found that several lncRNAs have been shown to be deregulated in ovarian cancer and the specific mechanism of their enrollment in ovarian cancer has been defined for a few of them. In addition, expression profiling has revealed an association between lncRNAs and patients' survival, metastasis potential as well as treatment response
Conclusions: Expression profiling as well as methylation analysis of lncRNAs in ovarian cancer may lead to the identification of novel biomarkers that can help in the classification of patients based on prognosis and treatment response
Subject(s)
Humans , Women , RNA, Long Noncoding , Carcinogenesis , Biomarkers, TumorABSTRACT
Background: Colon cancer-associated transcript 2 [CCAT2] is a newly recognized IncRNA transcribed from the 8q24 genomic region. It functions as an oncogene in various types of cancers including breast cancer, in which it affects Wnt/p-catenin pathway. Previous studies have shown a putative interaction between this IncRNA and MYC proto-oncogene
Methods: In the current study, we evaluated the expression of CCAT2 in breast cancer tissues with regards to the expression of its target MYC. In addition, we assessed the relationship between CCAT2 and MYC expression levels in tumor tissues and the clinical prognostic characteristics of breast cancer patients
Results: MYC expression levels were significantly up-regulated in tumor tissues compared with adjacent non-cancerous tissues [ANCTs], while such analysis showed no statistically significant difference between these two tissue types in CCAT2 expression. Starkly increased CCAT2 gene expression levels were found in 12/48 [25%] of cancer tissue samples compared with their corresponding ANCTs. Furthermore, significant inverse correlations were found between CCAT2 expression and stage, as well as lymph node involvement. Besides, a significant inverse correlation was found between the relative MYC expression in tumor tissues compared with their corresponding ANCTs and disease stage
Conclusions: These results highlight the significance of MYC and CCAT2 expressions in the early stages of breast cancer development and suggest a potentially significant role for CCAT2 in a subset of breast cancer patients, which could be applied as a potential therapeutic target in these patients